The museum was created in 1761 and is set in the Santo Cáliz Chapel of Valencia Cathedral.
It has two large canvasses painted by Goya in 1799, depicting San Francisco de Borja. It also has 15th and 16th-century paintings, with works by Rodrigo de Osona, Yanez de la Almedina, Vicente Masip and Juan de Juanes. Highlights of the sculptures on display include original pieces from the 14th-century Gothic Apóstoles door, and the Christ of the Good Death by Alonso Cano. You can also see the processional monstrance and other items in precious metals, such as chalices and reliquaries from the 14th and 15th centuries.