Brookville is a small city in northwestern Montgomery County, Ohio, United States. The population was 5,884 at the 2010 census, an increase from 5,289 in 2000. It is part of the Dayton Metropolitan Statistical Area. Brookville was first established in 1832 when Warren Estabrook opened a general store just north of the present-day intersection of Main and Wolf Creek Streets. This small community began to grow in 1844 when the Wolf Creek Turnpike was opened from Dayton to Brookville. Brookville's early growth was a direct result of its relationship with road and rail. The early trails through Brookville were the result of the work by the men under the lead of General Anthony Wayne. In 1793 his men cleared old Indian trails for easier travel.