Jonesborough (formerly spelled Jonesboro for a period but then changed back to conform to the original colonial spelling at the time of its founding) is a town in and the county seat of Washington County, Tennessee, in the southeastern United States. The population was 5,975 at the 2010 census. It is Tennessee's oldest town. Jonesborough is part of the Johnson City Metropolitan Statistical Area, which is a component of the Johnson City–Kingsport–Bristol, TN-VA Combined Statistical Area – commonly known as the "Tri-Cities" region.
Appalachian Ghost Walks, a ghost tour company that specializes in the "haunted" history of the Appalachian Mountains, has established a ghost tour of Jonesborough's downtown historic district. The Jonesborough Repertory Theatre produces community theater and hosts educational workshops for aspiring actors. The current artistic director is Jonathan McCarter.
Jonesborough Public Schools are part of Washington County Schools. The Washington County School District has nine elementary schools, two middle schools and three high schools.