Jacobswoude is a former municipality in the western Netherlands, in the province of South Holland, now part of municipality Kaag en Braassem. Before the merger with Alkemade, the municipality covered an area of 41.37 square kilometers (25.71 mile²) of which 4.88 square kilometers (3.03 mile²) is water, mainly the lakes Braassemermeer and Wijde Aa. It consisted of the communities Bilderdam, Hoogmade, Leimuiden, Rijnsaterwoude, and Woubrugge, all located around the above mentioned lakes.
Jacobswoude is located in the center of the Groene Hart (Green Heart), the slightly less densely populated center area of the Randstad. Inhabitants of Jacobswoude depend on Alphen aan den Rijn, Leiden and Amsterdam for facilities not offered in the smaller villages. Most of its inhabitants commute daily to one of the larger cities in the region for work.