Hiyoshi Taisha shrine is a Shinto shrine located in Ōtsu, Shiga, Japan. The West Hall of Worship and the East Hall of Worship have been designated by the Agency for Cultural Affairs as National Treasures in the category shrines. This shrine heads the seventh largest shrine network in Japan, at about 4,000 shrines.
Hiyoshi Taisha was first recorded in Kojiki, written in the 8th century. In the Middle Ages, the Enryaku-ji temple influenced the shrine to include some Buddhist essence. The buildings of the shrine were burnt when Oda Nobunaga destroyed Enryaku-ji in 1571. The existing buildings were constructed in the last quarter of the 16th century.