The Johannesburg Botanical Garden is located in the suburb of Emmarentia in Johannesburg, South Africa. The gardens were established in 1964 as a large rose garden (becoming known locally as the "Rose Garden") and subsequently expanded to cover an area of around 125 hectares (1.25 km2). It is administered by Johannesburg City Parks.
The Emmarentia Dam is situated immediately to the east of the garden and shares its extensive acreage. One of the main attractions is the Rose Garden with over 10 000 roses.
A monument commemorating the 300th anniversary of the Hugenots in South Africa is located at the top of the rose garden.