Desfina (Greek: Δεσφίνα) is a town and a former municipality in the southern part of Phocis, Greece. Since the 2011 local government reform it is part of the municipality Delphi, of which it is a municipal unit. At the 2001 census, its population was 2,431. It is found in foot of mountain Kirfis in altitude 680m. and it occupies an extent of 200.000 str. roughly which is extended up to the Corinthian Gulf. Desfina is built amphitheatrically and is located about 200 kilometres of Athens, roughly 35 kilometres of Livadia, 16 kilometres of Antikyra, 30 kilometres of Amfissa, 18 kilometres of Itea and roughly 30 kilometres of Delphi and Arachova.
One of the most important rebels of the Greek revolution, Bishop Isaias of Salona, fought for the liberation of Desfina until his execution by the Turks on 22 April 1821 and encouraged Athanasios Diakos for the battle of Alamana. Also, Isaias gave the signal for the beginning of revolution of Central Greece at the region of Prophet Daniel. His residence is found in Desfina and has been converted to a museum. In Desfina there is the Monastery of Honest Precursor which was distinguished at the revolution on 1821 as well as the very important Byzantine monument, the temple of Brigadiers near to the church of Saint Haralampos that dates to the 12th century.