Hustopeče is a town in southern Moravia of the Czech Republic with 5,956 inhabitants. Hustopeče lies in the Břeclav District and is 25 km northwest of Břeclav.
Hustopeče was first mentioned in 1303. By the end of the 19th century, it was on the byline from Šakvice north to the Vienna-Bruno-Prague line. Until the end of the Second World War, most of its inhabitants were ethnic Germans.